Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs
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چکیده
منابع مشابه
Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs
We developed a simple, direct and cost-effective approach to search for the most likely target genes of a known microRNA (miRNA) in vitro. We term this method 'labeled miRNA pull-down (LAMP)' assay system. Briefly, the pre-miRNA is labeled with digoxigenin (DIG), mixed with cell extracts and immunoprecipitated by anti-DIG antiserum. When the DIG-labeled miRNA and bound mRNA complex are obtained...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2009
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkp274